The isolation of exosome from amniotic membrane mesenchymal cells, and effect of exosome in the ocular surface.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09811
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56060:Ophthalmology-related
• Research Institution: Tokyo Dental College
• Principal Investigator: Shimazaki Jun 東京歯科大学, 歯学部, 教授 (40170930)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 羊膜 / 細胞外小胞体 / 創傷治癒

Outline of Final Research Achievements

We reported that amniotic membrane-deraived fibroblasts (AMFs) have multi-differentiation potential, and AMF-supernatant (AMF-sup) is promoting corneal epithelial wound healing. In this study, extracellular vesicles (EVs) were isolated from AMF-sup, analyzed its characteristics, and compared to its effects in the rabbit corneal epithelial wound healing model. 　Vesicles isolated from AMF-sup are many　spherical vesicles about 156±5.9nm in diameter, 　expressed marker of EVs such as CD63, CD9, and TSG101. Application of EVs resulted in maintenance of the limbal epithelial phenotype and immature state in cultivated human corneal limbal epithelial sheets, and significantly promoted wound healing in rabbit corneal epithelium compared with the control. These data suggest that EVs isolated from AMF-sup is effective in maintaining the limbal epithelial phenotype and promoting corneal epithelial wound healing, which may be of value in ocular surface reconstruction.

Free Research Field

眼科学

Academic Significance and Societal Importance of the Research Achievements

羊膜中に存在する間葉系細胞由来エクソソームの眼表面上皮に対する効果を調べることで羊膜の持つ効果のメカニズムを解明することが可能である。これにより羊膜を用いたより効果的な眼表面疾患の治療を行うことが可能となると同時に、羊膜由来セクレトームを用いた難治性眼表面疾患に対する新しい治療法として期待される。