Biological analysis of smooth muscle cells in soft tissue vascular malformation

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09864
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Kobe University
• Principal Investigator: Nomura Tadashi 神戸大学, 医学部附属病院, 准教授 (30529566)
• Co-Investigator(Kenkyū-buntansha): 寺師 浩人 神戸大学, 医学部附属病院, 教授 (80217421) ; 榊原 俊介 神戸大学, 医学部附属病院, 特命講師 (50444592) ; 橋川 和信 名古屋大学, 医学系研究科, 准教授 (90403237) ; 大崎 健夫 神戸大学, 医学研究科, 非常勤講師 (30444582) ; 櫻井 沙由理 (大澤沙由理) 神戸大学, 医学部附属病院, 非常勤講師 (20594534) ; 高須 啓之 山口大学, 医学部附属病院, 准教授 (40566022)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 静脈奇形 / 組織 / 硬化療法

Outline of Final Research Achievements

Surgical specimens of vascular malformations of the lip (VM) collected were examined immunohistochemically. Non-cystic sites were 85% in the combined sclerotherapy group and 80% in the surgery alone group. Collagen fibre sites were 49.9% in the former group and 36.8% in the latter, elastic fibre sites were similarly 25.8% and 27.7%, and muscle fibre sites were 29.6% and 13.9%. The lack of change in volume on pre- and post-treatment MRI suggested that replacement with fibrotic tissue occurred after sclerotherapy, as collagen fibres increased in VMs after sclerotherapy compared with those without. The cell culture of 'vascular smooth muscle cells' was mainly carried out, and immunohistological investigations and the proliferative capacity of the cells were confirmed by MTT assay to establish the technique for cell culture methods.

Free Research Field

形成外科学

Academic Significance and Societal Importance of the Research Achievements

臨床で頻用される静脈奇形に対する硬化療法の効果について、これまで作用機序に関する検討はあったものの、形態並びに組織学的な検討はこれまでなされておらず、本研究成果は、画期的と考えられる。臨床における今後の硬化療法の適応、治療回数ならびに摘出術への変更に関して、学術的に基本となる基礎研究であり、同疾患を治療する医療者や患者にとって有意義な結果といえる。