Development of a novel fat grafting method with ADSC spheroid

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09866
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Okayama University
• Principal Investigator: Nakagiri Ryoko 岡山大学, 大学病院, 助教 (10633753)
• Co-Investigator(Kenkyū-buntansha): 岩井 良輔 岡山理科大学, フロンティア理工学研究所, 講師 (60611481) ; 渡部 聡子 岡山大学, 医学部, 客員研究員 (20379803)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 脂肪組織由来幹細胞 / 細胞凝集塊 / スフェロイド / 成長因子 / 脂肪移植

Outline of Final Research Achievements

Fat grafting with Adipose tissue-derived stem cells (ASCs) have been reported to have a good engraftment rate. In this study, we aimed to further improve the engraftment rate of transplanted fat by adding cell aggregates of ASCs to the transplanted fat.
We created cell aggregates to create spherical cell aggregates (spheroids) of different sizes, fiber-like cell aggregates, and mesh-like cell aggregates. We succeeded in fabricating mesh-like cell aggregates with 9-lattice structures using the guided self-aggregation technique (CAT).
In addition, it was confirmed that the amount of growth factor produced differs depending on the cell morphology. By co-cultivating ASC with fat, we were able to confirm the maintenance of the fat mass, but we could not demonstrate the usefulness of the ASC aggregate.

Free Research Field

形成再建外科

Academic Significance and Societal Importance of the Research Achievements

自己凝集化誘導技術（CAT）を用いてメッシュ状の細胞凝集塊の作製に成功した。細胞がメッシュ状の凝集塊の形態をとることにより細胞生着率の向上が期待できる。これは、脂肪以外の移植医療でも有用な可能性がある。細胞生着率が向上すれば細胞移植医療でさらなる効果が期待できる。