Elucidation of mechanism for periodontal regeneration via exosome and application for periodontal regenerative therapy

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09975
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Iwata Tomoyuki 広島大学, 病院(歯), 助教 (30418793)
• Co-Investigator(Kenkyū-buntansha): 水野 智仁 広島大学, 医系科学研究科(歯), 教授 (60325181)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 歯周組織再生 / 間葉系幹細胞 / 歯周靭帯細胞 / 歯肉線維芽細胞 / HDAC / ヒストンアセチル化

Outline of Final Research Achievements

On MSC transplantation as periodontal regenerative therapy, MSCs are regulated MSC function including cell differentiation by periodontal cells located at the transplantation site with direct contact or indirect contact via humoral factor. However, there are a lot of unknown points as to humoral factors secreted by periodontal cells, and, therefore, in this research, I have investigated the regulatory factor and regulatory mechanism for MSC function by humoral factor from periodontal cells.
By this research, it has been clarified that MSCs were affected in their HDAC1/HDAC2 expression, phosphorylation, and activity by periodontal humoral factor, then MSC osteogenesis was regulated.
In the future, I believe this achievement can result in the establishment of coordinated with periodontal cells and Effective periodontal regenerative therapy.

Free Research Field

歯周病学

Academic Significance and Societal Importance of the Research Achievements

本研究により、歯周組織構成細胞であるHPL cellsとHGFからの液性因子によって、MSCの骨分化が制御される際に影響を受ける因子が確定された。これにより、MSC細胞機能制御因子の中心を担う因子の一つが同定されたため、今後、これらの因子に対する制御因子の同定および相互作用因子の同定を行うことによって、これらを応用した歯周組織構成細胞と調和の取れた効果的な歯周組織再生治療法の確立につながることができ、更には、他の医療分野において応用されるMSCの細胞機能制御因子の同定につながると考えられるため、学術的意義および社会的意義は大きいと考えられる。