2022 Fiscal Year Final Research Report
Establishment of in situ tissue engineering approach using bioactive agent and various biomaterials for obtaining true periodontal tissue regeneration
| Project/Area Number |
20K10011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57040:Regenerative dentistry and dental engineering-related
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中村 利明 鹿児島大学, 医歯学域鹿児島大学病院, 講師 (60381183)
篠原 敬哉 鹿児島大学, 医歯学域歯学系, 助教 (30761647)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 歯周組織再生 / 骨移植材 / 塩基性線維芽細胞増殖因子 / 担体 / 足場材 / テイッシュエンジニアリング |
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| Outline of Final Research Achievements |
To evaluate periodontal wound healing of 1-wall intrabony defects treated with recombinant human fibroblast growth factor-2 (rhFGF-2) and beta-tricalcium phosphate (β-TCP), carbonate apatite (CO3Ap), or deproteinized bovine bone mineral (DBBM) in dogs. The stability of rhFGF-2 adsorbed onto the bone substitutes was evaluated by ELISA.Significantly higher amount of rhFGF-2 was adsorbed to CO3Ap compared with β-TCP. The rhFGF-2/DBBM group demonstrated the highest amount of periodontal tissue regeneration. The rhFGF-2/DBBM group yielded significantly greater formation of new attachment than the rhFGF-2 group. New bone area in the rhFGF-2/DBBM was significantly greater than that in the rhFGF-2, rhFGF-2/β-TCP and rhFGF-2/CO3Ap groups. The present data indicate that DBBM seems to be a suitable carrier for rhFGF-2 and the rhFGF-2/DBBM treatment promotes favorable periodontal regeneration compared with rhFGF-2, rhFGF-2/β-TCP and rhFGF-2/CO3Ap treatments in 1-wall intrabony defects.
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| Free Research Field |
歯周病学分野
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| Academic Significance and Societal Importance of the Research Achievements |
本研究結果により, bFGFは併用する骨移植材の特性により各々の相性（affinity)が異なることが示唆された。なお歯周組織再生療法に用いる生体材料は足場材としての役割でなく, 生理活性物質の担体としての役割を併せもつものが理想的であることが示され今後, 理想的な生体材料の開発にも繋がる知見が得られた。さらには世界に先駆けて, 本邦におけるbFGFと骨移植材を併用した歯周組織再生治療の治療指針のエビデンスを提示できた点において非常に意義深いものと考えられる。
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