Establishment of the new diagnosis biomarkers for metal allergy by analyzing peripheral blood miRNA expression

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K10038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: The University of Tokushima
• Principal Investigator: HOSOKI Maki 徳島大学, 大学院医歯薬学研究部(歯学域), 講師 (10228421)
• Co-Investigator(Kenkyū-buntansha): 大島 正充 徳島大学, 大学院医歯薬学研究部(歯学域), 准教授 (00548307) ; 井上 美穂 徳島大学, 大学院医歯薬学研究部(歯学域), 助教 (20271059) ; 三好 圭子 徳島大学, 大学院医歯薬学研究部(歯学域), 准教授 (20304537) ; 松香 芳三 徳島大学, 大学院医歯薬学研究部(歯学域), 教授 (90243477)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 金属アレルギー / マイクロRNA

Outline of Final Research Achievements

WThe study was conducted with the cooperation of healthy volunteers and outpatients with dental metal allergy who gave informed consent.
Peripheral blood samples were collected before, 72 hours after, and 1 week after the patch test for microRNA analysis. MicroRNA analysis was performed using a high-sensitivity DNA chip (3D-Gen, Toray Industries, Tokyo, Japan) with excellent detection sensitivity, and microRNAs that could be candidate biomarkers for diagnosis and treatment were investigated. In addition, to explore the trends of patients with dental metal allergy, we compiled a database of age, sex, symptoms, patch test results, positive metal element types, and allergic symptoms of patients who visited outpatient dental metal allergy clinics, and conducted an epidemiologic study.

Free Research Field

歯科補綴学

Academic Significance and Societal Importance of the Research Achievements

網羅的なmicroRNA解析の手法は，癌研究に多用されており，金属アレルギーについては，このような研究は少ない，研究を展開することにより，他の研究との関連も得られると考える．