The function of RNA binding proteins and messenger ribonucleoprotein granules in oral squamous cell carcinoma

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K10133
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Kuroshima Takeshi 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (00610669)
• Co-Investigator(Kenkyū-buntansha): 東野 史裕 北海道大学, 歯学研究院, 准教授 (50301891) ; 北村 哲也 北海道大学, 医学研究院, 客員研究員 (00451451)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 口腔がん / 扁平上皮癌 / 頸部リンパ節転移 / Sam68 / RNA結合タンパク質

Outline of Final Research Achievements

1. We analyzed the biological function of Sam68, which is one of the RNA binding proteins (RBP), in oral squamous cell carcinoma. Results suggests that Sam68 may contribute to cervical lymph node metastasis due to the regulation of epithelial mesenchymal transition via modulating Vimentin expression. 2. Proteome analysis using cisplatin sensitive and resistant OSCC cells was performed. One hundred fifty-one RBPs were profiled in differentially expressed proteins between two groups. These RBPs were concentrated in the process of RNA processing and splicing in the enrichment analysis result.

Free Research Field

外科系歯学、病態科学系歯学

Academic Significance and Societal Importance of the Research Achievements

1.本研究の成果は、Sam68が口腔扁平上皮癌における頸部リンパ節転移の新たな予測マーカーや、新規治療法の標的となる可能性を示している。
2.本研究の結果は、口腔扁平上皮癌におけるシスプラチン抵抗性に関与するRNPおよびRNP granulesの機能解明の基盤となる可能性を示している。