Bone regeneration by ultrasonic treatment with electrized water for dense bone

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K10186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: MURATA Masaru 北海道医療大学, 歯学部, 教授 (00260662)
• Co-Investigator(Kenkyū-buntansha): 根津 尚史 北海道医療大学, 歯学部, 准教授 (40264056) ; 斎藤 隆史 北海道医療大学, 歯学部, 教授 (40265070) ; 建部 廣明 北海道医療大学, 歯学部, 講師 (40638293)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 緻密骨 / 硬化骨 / 超音波 / 電解水 / 骨誘導 / 骨再生

Outline of Final Research Achievements

Ultrasonically scratched dense bone plate treated with acidic electrolyzed water (AEW)induced bone at 2 weeks after the graft in rat subcutaneous tissues. The bone-induced region was in the compartment (space like closed room) of AEW bone. The 3D space contributes maintenance of growth factors's concentration and its increase. In addition, the bone-demineralization by acidic electrolyzed water(AEW) expose collagen fibers (RGD sequence). We believe mesenchymal cells attach to the exposed collagen fibers as cell anchorage, and react with bone morphogenetic proteins from bone matrix, proliferate and differentiate into osteoblasts.
On the other hand, fresh dense bone, perforated dense bone and dense bone plate treated with AED did not induce bone and cartilage until 8 weeks.

Free Research Field

骨再生

Academic Significance and Societal Importance of the Research Achievements

新鮮骨移植がゴールドスタンダードと信じられているが加工処理骨の方が骨誘導能が高いことが明らかになった．特に緻密硬化骨は構造と骨質が悪いため，骨代謝・リモデリングに調和して組み込まれない．緻密硬化骨で骨再生を早期に起こすには超音波スケーラーで骨構造の改質と酸処理でコラーゲンを露出させる処理のコンビネーションが効果的である．
新鮮骨神話が崩れ，２１世紀はチェアーサイドでの本技術による加工骨が移植骨のゴールドスタンダードになるであろう．