2023 Fiscal Year Final Research Report
Reconstruction of extensive mandibular bone defects using dedifferentiated adipocyte-derived exosomes.
| Project/Area Number |
20K10195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57060:Surgical dentistry-related
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| Research Institution | Osaka Dental University |
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Principal Investigator |
KUBO Hirohito 大阪歯科大学, 歯学部, 講師 (70388362)
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| Co-Investigator(Kenkyū-buntansha) |
橋本 典也 大阪歯科大学, 歯学部, 教授 (20228430)
本田 義知 大阪歯科大学, 歯学部, 教授 (90547259)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | エクソソーム |
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| Outline of Final Research Achievements |
Flow cytometry of the generated cells was performed and was negative for both the Th ligand marker CD28 and the vascular epithelial marker CD140. The stem cell marker CD90 was positive. The results showed that the cells prepared using ceiling culture were dedifferentiated adipocytes. Nanocytometric results showed an average particle size of 122-140 nm and a concentration in the specimen of 4.30-4.74 (10X9 particles/ml).TEM showed particles with a lipid bilayer of approximately 100 nm in diameter.DFAT-Ev was found to be nanoflow cytometry RT-PCR showed differential expression of osteoblast differentiation markers with DFAT-Ev.
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| Free Research Field |
再生歯学
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| Academic Significance and Societal Importance of the Research Achievements |
今回、我々は細胞移植による広域の骨欠損治療のハードルとなっている二つの原因、①純度の高い（homogeneousな）細胞集団の確保、および②移植局所の血管新生の改善を研究ターゲットとした新規広域骨組織再建法の開発を目的とした研究を計画した。すなわち、骨の再建に必須となる大量の間葉系幹細胞の確保のために脂肪を細胞供給源としたDFATsを作製し、さらに移植局所の血管新生の改善にはDFATsエクソソームを用いる。以上、これまでの細胞移植の問題点を解決し、大型の骨再生を誘導する方法を見出すことを本研究課題によって可能となった。
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