Elucidation of the calcium channelopathies affecting oral health-related QoL.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K10228
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Kyushu University
• Principal Investigator: Haruyama Naoto 九州大学, 歯学研究院, 准教授 (70359529)
• Co-Investigator(Kenkyū-buntansha): 寺尾 文恵 九州大学, 歯学研究院, 助教 (10510018)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ストア作動性カルシウム流入 / STIM1 / カルシウムイオン / 唾液腺 / QoL / 皮膚

Outline of Final Research Achievements

In this study, we generated epithelial-specific Stim1/2 mutant mice and performed phenotypic analysis to elucidate the pathogenesis of calcium channelopathies that affect oral health-related QoL. The results showed that Stim1/2 mutant mice not only had decreased volume of salivary secretion but also had altered chloride ion concentrations in saliva. These results suggest that impaired SOCE may affect chloride ion concentrations and alter primary salivary induction, resulting in decreased salivary secretion. In addition, abnormal SOCE also altered epidermal barrier function in Stim1/2 mutant mice, suggesting that calcium ion is critical for epidermal homeostasis.

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

我々が作出したマウスは、対象となる唾液腺および皮膚組織におけるカルシウムチャネロパチーの病態解明に向けて利用可能な有用なモデルとなりうることが確認できた。またこのモデルを解析して得られた成果は、カルシウムチャネロパチーの中でも特に解明が立ちおくれている、ストア作動性カルシウム機構の異常がもたらす、唾液腺機能異常による口腔乾燥症や、口腔上皮を中心とした上皮分化・創傷治癒の役割について、その理解と治療につながる有益な情報になると考えられた。