2022 Fiscal Year Final Research Report
Acetaminophen-induced acute liver injury and inflammasome
| Project/Area Number |
20K10561
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 58040:Forensics medicine-related
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | アセトアミノフェン / 肝障害 |
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| Outline of Final Research Achievements |
Acetaminophen (APAP), which is widely used as an antipyretic analgesic, is easily available, and its accidental poisoning as well as suicides have become a problem. In this study, we focused on the inflammasome and analyzed the inflammasome status in acetaminophen-induced liver injury using mice. Total proteins were extracted from liver tissues, and NLRP3, NLRP1, NLRC4, and AIM2, which are molecules involved in signal recognition, were detected by western blotting, and changes over time were examined. NLRP1 and IL-18 were markedly upregulated at 6 and 24 hours after APAP administration. These molecules may be indicators of APAP liver injury.
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| Free Research Field |
実験病理学
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| Academic Significance and Societal Importance of the Research Achievements |
アセトアミノフェン中毒の病態におけるインフラマソームの様態を詳細に解析し，アセトアミノフェン肝障害発症メカニズムを解明することができれば，従来の化学分析に加えて，インフラマソームがアセトアミノフェン中毒死判断のための新たな分子指標となるものと考えられる．その結果，より客観的かつ正確な死因判定が可能となり，法医中毒学の新たな展開につながると考えられる．
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