Does the obesity affect the relationship between serum haptoglobin and cholesterol levels?

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K10571
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58040:Forensics medicine-related
• Research Institution: Kurume University
• Principal Investigator: Koda Yoshiro 久留米大学, 医学部, 教授 (90231307)
• Co-Investigator(Kenkyū-buntansha): 副島 美貴子 久留米大学, 医学部, 准教授 (80279140)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: ハプトグロビン / コレステロール / 関連解析 / 肥満

Outline of Final Research Achievements

Haptoglobin (HP) has genetic polymorphisms that affect its blood levels in addition to fluctuations in concentration due to hemolysis or disease. We examined whether obesity (BMI value) affects the relationship between blood HP and cholesterol levels in a Japanese population, as observed in previous study, and finally to understand the role of HP in cholesterol metabolism and the effect of obesity in this study. First of all, we aimed to establish methods both for HP quantification and genotyping of polymorphisms suitable for a large number of small-volume samples. We were able to develop a method for simultaneous determination of two key polymorphisms, while the protein quantification method is still under investigation. We plan to achieve our goal by using these methods.

Free Research Field

法医遺伝学

Academic Significance and Societal Importance of the Research Achievements

複数のたんぱく質や遺伝子などを対象とした研究では、一度の反応で複数の遺伝子座を調べることができればサンプリングのミスの軽減につながり、試薬、検体、操作時間を節約することが可能となる。今回開発した一回のPCR反応で融解曲線解析とエンドポイント法を利用した遺伝子型判定法についてはこれまでに報告がなく恐らく最初の報告であり、本研究成果を参考に他の遺伝子多型のマルチプレックスな解析方法が開発される可能性が期待される。