2022 Fiscal Year Final Research Report
S-glutathionylation on C/EBPb stimulates adipogenesis and obesity
| Project/Area Number |
20K11528
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 59040:Nutrition science and health science-related
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 蛋白質S-グルタチオン化 / 肥満 / C/EBPβ / レドックス / Glrx |
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| Outline of Final Research Achievements |
In this study, we confirmed that glutaredoxin knockout in 3T3L1 cells enhances S-glutathionylation after induction of adipogenesis and promotes adipogenesis. Furthermore, C/EBPβ, a transcription factor involved in adipocyte differentiation, was found to be increased. Mass spectrometry analysis confirmed that C/EBPβ is glutathionylated, C/EBPβ is SUMOylated by PIAS1, a SUMO E3 ligase, and is subsequently ubiquitinated and degraded into ubiquitin proteasomes. S-glutathionylation of C/EBPβ is important for adipogenesis.
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| Free Research Field |
循環器内科
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| Academic Significance and Societal Importance of the Research Achievements |
蛋白S-グルタチオン化の亢進が脂肪合成を促進することを解明した。またＣ／ＥＢＰβが新規の脱グルタチオン化酵素であることを発見した。臨床検体を用いて、脱グルタチオン化酵素Ｇｌｒｘが肥満のマーカーであることを報告した。
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