Control of protein expression dynamics by using microfluidic and optogenetic technology

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K12050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 62010:Life, health and medical informatics-related
• Research Institution: Kogakuin University
• Principal Investigator: Kaneda Shohei 工学院大学, 工学部, 准教授 (10542467)
• Co-Investigator(Kenkyū-buntansha): 磯村 彰宏 京都大学, 高等研究院, 連携助教 (70512466)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: マイクロ流体 / 光遺伝学技術 / タンパク質発現ダイナミクス

Outline of Final Research Achievements

An experimental platform was developed using a microfluidic device capable of precisely adjusting two inputs: the concentration conditions of a low-molecular weight (LMW) compound and light stimulation conditions. This platform aimed to control the dynamics of protein expression (output). The concentration conditions in the microchannels as cell culture chambers, were adjusted by controlling the flow rate of syringe pumps. The light stimulation conditions for the chambers were adjusted by controlling the blue LED light source. Additionally, a feedback system was implemented into the platform to monitor the protein expression level and adjust the two inputs.
Furthermore, C2C12 cells to visualize of the dynamics were generated, which could express RFP through light stimulation and suppress its degradation rate in a concentration-dependent manner using the compound. In conclusion, the technical foundation was established to elucidate the significance of these dynamics for cell functions.

Free Research Field

マイクロ流体デバイス

Academic Significance and Societal Importance of the Research Achievements

本研究の成果である，マイクロ流体デバイスを中心とした低分子化合物濃度条件と光刺激条件の2入力を精密に調整可能な実験プラットフォームは，タンパク質発現ダイナミクスを人工的に制御する際の自由度を増大することができます．これにより，タンパク質発現ダイナミスクが，増殖や分化などの細胞機能制御へ果たす役割を解明することに役立つことが期待されます．