Detection of blood biomarkers for Alzheimer's disease by horn-type ESR

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K12626
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Oita University
• Principal Investigator: Kamida Tohru 大分大学, 医学部, 客員研究員 (90315333)
• Co-Investigator(Kenkyū-buntansha): 小林 正 大分大学, 理工学部, 名誉教授 (30100936)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ADの早期診断方法 / ESRバイオマ－カ－検出キット / 磁場/周波数掃引QD法 / コルゲートホーン型ESR装置 / Sパラメータ計測ESR手法

Outline of Final Research Achievements

We developed free radical-related biomarkers in presymptomatic Alzheimer's disease (AD) peripheral blood samples and attempted to quantify them using a novel frequency sweeping electromagnetic horn ESR device. Due to COVID-19, we were unable to produce an ESR biomarker detection kit. On the other hand, a magnetic field/frequency sweep QD method corrugated horn type ESR device was devised, and in terms of hardware, a large signal increase and superiority in measurement were obtained by HEMT and corrugated horn. On the software side, integration and FFT filter processing reduced the noise width during HEMT amplification and improved the sensitivity by about 10 times in the S/N ratio. The S-parameter measurement ESR method was also examined, and a basic development method was established for practical use.

Free Research Field

ESR計測の医学応用

Academic Significance and Societal Importance of the Research Achievements

電磁ホーン型ESR装置は、バイオマ－カ－検出キットごとESR計測が可能である。しかしながら既存の装置では測定感度が十分でなく、実用的でなかった。本研究の成果として、従来の10倍程度に感度が向上した。今後、ESRバイオマーカー検出キットが開発されれば、本手法が、アルツハイマー病の早期発見の一指標となる可能性があり社会的意義は大きい。