Classification of osteoblast-derived extracellular vesicles by glycans and cellular responses

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K12645
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: Kyoto University
• Principal Investigator: Shimoda Asako 京都大学, 医学研究科, 特定研究員 (90712042)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 細胞外小胞 / 糖鎖解析 / レクチンマイクロアレイ

Outline of Final Research Achievements

We focused on surface glycans as indicators for classifying extracellular vesicles (EVs), which are nano- to micro-sized particles with heterogeneous properties. In this study, EVs from mesenchymal stem cells (MSCs) and osteoblast differentiated MSCs were collected and their glycan patterns were analyzed using lectin microarray and single particle analysis. The glycan patterns were different in accordance with the cell origin and types of vesicles (EVs or matrix vesicles which play important roles in bone mineralization). Future studies are needed to fully understand the interaction between EVs and cells depending on EV surface glycans.

Free Research Field

バイオマテリアル

Academic Significance and Societal Importance of the Research Achievements

細胞外小胞の糖鎖解析に関する報告例はその他の構成成分であるタンパク質、脂質、核酸に比べて少なく、レクチンマイクロアレイ法による解析は糖鎖のパターンを少量のサンプルで網羅的に解析する手法として有用である。さらに、一粒子解析を行うことでより詳細な評価が可能となり、細胞外小胞の不均一性の解明やバイオマーカーの特定に応用できると考えられる。