Development of sensitive and naked-eye biomarker detection toward cancer point-of-care diagnosis

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K12697
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90130:Medical systems-related
• Research Institution: Juntendo University
• Principal Investigator: ISHIHARA Ryo 順天堂大学, 医学部, 准教授 (30633507)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 表面機能化自律駆動マイクロチップ / UVグラフト重合法 / 細胞外ベシクル / microRNA / バイオマーカー検出 / がん / その場診断

Outline of Final Research Achievements

In this study, we aimed to establish a point-of-care diagnostic method for various severe diseases, including cancer, by developing a simple and highly sensitive detection method for extracellular vesicles and microRNAs, which are anticipated as biomarkers. By incorporating constricted sections within the microchannels and chemically modifying the inner surfaces of the portable "surface-functionalized power-free microchip" developed by the applicant, we achieved more efficient biomarker detection. Consequently, we succeeded in improving the detection sensitivity by 2 to 3 orders of magnitude.

Free Research Field

バイオマテリアル

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた，細胞外ベシクルの検出感度は唾液や血液といった生体サンプルで実現することができればがん診断の実現が期待できる値である。しかし生体サンプル由来の夾雑物の影響で感度が低下してしまうことも考えられ，また血清など生体サンプルを流すと一定の頻度でマイクロ流路が詰まってしまうという課題が残っている。今後これらの課題を解決し，さまざまな疾患をその場診断できる手法を確立したい。