2021 Fiscal Year Final Research Report
Creation of Clock Tissue Engineering usin by Microfluidics
| Project/Area Number |
20K15099
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | 幹細胞 / 時計遺伝子 / 熱刺激 / マイクロ流体デバイス |
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| Outline of Final Research Achievements |
Functionalization of human pluripotent stem cell-derived hepatocytes (hPSC-hep) is important in the study of liver and drug discovery, but this has not yet been achieved. In this study, I focused on the biological clock as a functionalization method and aimed to elucidate the clock oscillation mechanism of hPSC-Hep using microfluidic devices (μFD) to make hPSC-Hep more functional and mature. In this study, 1) I succeeded in developing a microfluidic device capable of precise time factor administration, 2) I found that the oscillation of clock genes in the differentiation process of hepatocytes starts after differentiation into hepatoblasts, and 3) I found that temperature change promotes the function of hepatocytes.
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| Free Research Field |
組織工学
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| Academic Significance and Societal Importance of the Research Achievements |
hPSC-Hepの機能化はこれまでに多くの研究者が取り組んできたが、機能を成熟化させることは困難であった。hPSCからの肝臓分化誘導においては、多くの研究が成長因子や細胞の混合、オルガノイド形成などからアプローチであるが、時計遺伝子の関連性に関しては正しく「No man’s land」状態である。本研究は、この時計遺伝子と分化誘導・機能発現の重要性にいち早く気づいた研究であり、正しく時間を制御することが可能なマイクロ流体デバイスと融合させることで機能的なhPSC-Hepの作製が可能となり、hPSC-Hepの実用化に大きく貢献できることが期待される。
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