2021 Fiscal Year Final Research Report
Development of an intelligent bio-Raman analysis approach
Project/Area Number |
20K15315
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 34020:Analytical chemistry-related
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Research Institution | Keio University |
Principal Investigator |
Yasuda Mitsuru 慶應義塾大学, 医学部(信濃町), 助教 (20742307)
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Project Period (FY) |
2020-04-01 – 2022-03-31
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Keywords | ラマン分光 / 光学干渉 / スペクトル |
Outline of Final Research Achievements |
In order to develop a high-speed Raman spectrum measurement technique, we fabricated an optical interference mirror (OIM) slide consisting of a transparent Al2O3 ultrathin film on an Ag slide. It was suggested that the Raman scattering light with the OIM slide is enhanced by the strong scattering of the excitation light. Therefore, when the OIM slide providing the high Raman enhancement was applied to Raman spectrum measurement of cultured human cells, a very strong Raman signal was observed with the OIM slide compared to a common glass slide. Thus, we were able to develop a high-speed Raman spectrum measurement technique that can shorten the measurement time.
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Free Research Field |
分析化学
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Academic Significance and Societal Importance of the Research Achievements |
細胞のラマンスペクトル測定は,極めて微弱なラマン散乱光が原因で,非常に長い時間を要することが問題となっていた。そのようななか,本研究ではナノ薄膜干渉基板により,ヒト培養細胞からのラマンシグナルを増強したことで,高速ラマンスペクトル測定が可能な技術を開発した。本技術は,短い時間で起きる生化学反応の追跡のみならず,生体分子の高感度な検出を可能にすることから,生物医学研究のさらなる発展に貢献することが期待される。
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