2021 Fiscal Year Final Research Report
Studies on novel feedback mechanism of methionine biosynthetic enzyme
| Project/Area Number |
20K15437
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Fukui Prefectural University (2021)
University of Shizuoka (2020) |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | メチオニン / 生合成 / フィードバック阻害 / 活性制御 / タンパク質間相互作用 |
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| Outline of Final Research Achievements |
In this study, we focused on the regulation of the enzymatic activity involved in the methionine biosynthesis and revealed that the uncharacterized protein MetW from Pseudomonas aeruginosa (PaMetW) regulated the succinyl-transferase activity of MetX from P. aeruginosa (PaMetX). In addition, we also clarified that the alanine substitution of the glycine-rich sequence of the PaMetW relieved the feedback inhibitory effect by the S-Adenosyl-L-homocysteine (SAH).
Furthermore, we demonstrated that the N-terminal region of PaMetW and the C-terminal region of PaMetX were significant for the interaction between PaMetW and PaMetX. This result coincided with the complex model of PaMetW and PaMetX generated by AlphaFold2.
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| Free Research Field |
応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、緑膿菌などの細菌が有する機能未知タンパク質MetWが必須アミノ酸であるメチオニンの生合成酵素MetXの活性制御を担うタンパク質であることを明らかにし、MetWとMetXとの相互作用に重要な領域を明らかにした。本研究成果は、メチオニン生合成においてフィードバック阻害による活性制御機構に多様性が存在することを示しただけでなく、今後のメチオニン関連代謝産物の発酵生産やMetWを標的とした抗菌剤開発への寄与も期待される。
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