Search for pesticide lead compounds using endophytic fungi on carnivorous plants.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K15531
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Kitasato University
• Principal Investigator: sakai kazunari 北里大学, 大村智記念研究所, 助手 (90760075)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: P. oryzae / D. subsupicatus / 微生物由来農薬 / 食虫植物由来微生物

Outline of Final Research Achievements

The applicant's goal was to use microorganisms as a search source to find microorganisms that produce antimicrobial substances against plant pathogens and to obtain antimicrobial substances. I believed that by introducing eco-toxicity testing as part of the screening process, he would be able to search for environmentally friendly pesticide seed compounds. In the first year, fungi were isolated from previously collected mousseline moss. Forty-six fungal strains were isolated, three of which may be new species. Subsequently, the isolated fungi and the microbial library owned by Kitasato University were evaluated for antimicrobial activity against P. oryzae. An environmental toxicity test against D. subspicatus was constructed and evaluated against the samples that showed antimicrobial activity. As a result, five samples were selected and purified to isolate hymeglusin and antimycin, respectively.

Free Research Field

天然物化学

Academic Significance and Societal Importance of the Research Achievements

本研究により見出されたvalinomycin、mocimycin及びhymeglusinは今までに農薬用抗菌剤として上市されてこなかった作用機序が予想されていることからこれら化合物の誘導体またはこれらの作用点を決め、その作用点に対して特異的に作用する異なる化合物の提示にも繋がることが予想される。