2022 Fiscal Year Final Research Report
Exploration of shrimp hematopoietic stem cells using single-cell mRNA analysis technology with microfluidic devices
Project/Area Number |
20K15603
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 40040:Aquatic life science-related
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Research Institution | Tokyo University of Marine Science and Technology (2021-2022) Tokyo University of Agriculture and Technology (2020) |
Principal Investigator |
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | シングルセル解析 / Drop-seq / 非モデル生物 / クルマエビ / 血球細胞 / ウイルス感染 / WSSV |
Outline of Final Research Achievements |
The result of single-cell analysis of kuruma shrimp hemosytes showed that hemocytes were classified into multiple cell groups, and marker genes specific to each group were obtained. Since the transcription amount of immune-related genes differed depending on the cell group, it was estimated that the immune function differed for each group. White spot syndrome virus infection increased or decreased the proportion of specific cell groups, particularly decreasing the proportion of cell groups expressing antibacterial peptides. In addition, multiple marker genes that can objectively classify kuruma shrimp hemocytes regardless of virus infection status were identified, and their mRNA expression patterns were successfully observed.
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Free Research Field |
魚介類免疫学
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Academic Significance and Societal Importance of the Research Achievements |
本研究成果により、クルマエビのようなモデル生物と比較して細胞の分類に課題がある養殖対象魚介類の細胞でも、網羅的シングルセルmRNA解析を実施することで、体を構成する細胞の図鑑(細胞アトラス)を構築可能であることを明らかにしました。 また、ウイルス感染時に割合が減少する血球細胞集団が判明したため、この集団を増やす、もしくは、減らさないようにする飼育法や飼料添加物を開発することで、クルマエビ類養殖で問題となっているウイルス病の克服に繋がると期待されます。
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