Developmental characterization of polyploid embryos in vitro using three stem cell lineages derived from blastocysts.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K15664
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Yamaguchi University
• Principal Investigator: Imai Hiroyuki 山口大学, 共同獣医学部, 助教(テニュアトラック) (60826155)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 初期胚 / 幹細胞 / 倍数性 / 多倍体

Outline of Final Research Achievements

Vertebrates have evolved through whole-genome duplication, which has helped them acquire new functional gene sets and adapt to their environment. Polyploidization, which is generally beneficial for eukaryotes, is not present in mammals due to embryonic development abnormalities. This study aims to establish three stem cell lineages and profile the effects of ploidy changes on mammalian embryonic development. Optimal timing and fusion protocols were established for reduplication through time-lapse observation of polyploid embryos. The establishment of embryonic stem cell and trophoblast stem cell lineages was successful for polyploid lineages, with colonies exhibiting a naive-type colony morphology and showing alkaline phosphatase-positive screening markers. In summary, we established and analyzed a ploidy series of embryonic stem cells and trophoblast stem cells.

Free Research Field

獣医学

Academic Significance and Societal Importance of the Research Achievements

本研究では、哺乳類の多倍体化拒否機構の原因を探るための細胞株の樹立とスクリーニングを行なった。その結果、倍数性シリーズで複数の幹細胞株の樹立に成功した。これらの細胞株はバイオリソースとして将来にわたって活用できる。またスクリーニングの結果、倍数性変動に伴って変動する遺伝子群を見出しており、多倍体化拒否機構を紐解くマイルストーンへ到達したと言える。