Establishment of a molecular clone of feline morbillivirus by reverse genetics system and elucidation of its intracellular entry mechanism

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K15685
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Osaka Medical and Pharmaceutical University
• Principal Investigator: Sakaguchi Shoichi 大阪医科薬科大学, 医学部, 助教 (20815279)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: ネコモルビリウイルス

Outline of Final Research Achievements

The plasmids encoding the viral genome, the N gene, the P gene, and the L gene were prepared for the "establishment of a molecular clone of feline morbillivirus by reverse genetics system." Also, to set up the same conditions for transfection as actual virus infection, the expression levels of each gene during infection with wild-type virus were examined. In addition, we established a method for identifying cellular factors involved in viral infection and a system for searching for closely related viruses and summarized the results in two papers, respectively.

Free Research Field

ウイルス学

Academic Significance and Societal Importance of the Research Achievements

本邦では900万頭近いネコが飼育されており、近年は新規飼養頭数が増加している。ネコは泌尿器疾患の罹患率が比較的高く、その治療・ケアは飼育の負担となっている。ネコモルビリウイルス感染はネコの尿細管間質性腎炎との関与が示唆されていることから、このウイルスを研究することは獣医療・飼い主にとって重要だと考えられる。本研究によりネコモルビリウイルスの性状がさらに明らかとなり、また近縁のウイルスが探索できるようになった。