2021 Fiscal Year Final Research Report
Elucidation of the physiological functions of chicken's paneth cells and intestinal epithelial cells in the intestinal crypt
Project/Area Number |
20K15694
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 42030:Animal life science-related
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Research Institution | Akita Prefectural University |
Principal Investigator |
Ito Ken 秋田県立大学, 生物資源科学部, 助教 (30818604)
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Project Period (FY) |
2020-04-01 – 2022-03-31
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Keywords | 鶏 / 腸上皮細胞 / パネート細胞 / 腸管陰窩 |
Outline of Final Research Achievements |
There are few reports on chicken paneth cells, and their localization and physiological function are unknown. Therefore, I tried to localize paneth cells in the chicken intestine by IHC, but it was difficult to detect them with the existing antibodies against paneth cell markers due to the weak signal and the strength of autofluorescence. Based on the above, we searched for a method for suppressing autofluorescence, and tried single-cell analysis for searching markers with strong signal intensity. As a result, although autofluorescence could be attenuated, it could not be completely suppressed and could not be effective against weak signals. In single-cell analysis, it was possible to isolate crypt-derived cells, but there was a problem in single cellization from the cell population.
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Free Research Field |
家畜栄養学
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Academic Significance and Societal Importance of the Research Achievements |
腸上皮細胞の一つであるパネート細胞は、腸管幹細胞に隣接しており、抗菌物質および成長因子を分泌することで腸幹細胞の保護および増殖・分化を制御している。近年では栄養素の対する応答への関与も示唆されている。しかし、鶏のパネート細胞に関する報告はほとんどなく、その局在および腸管細胞への関与についても不明である。鶏腸管内のパネート細胞の局在を明らかにし、生理学的役割を把握することで、栄養成分および病原体への応答機構が明らかとなり、生産性を高めた飼料、飼養手法の開発に繋がる。
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