Involvement of the localization of ER exit site in secretory function

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K15787
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: Wakayama Medical University
• Principal Investigator: Baba Takashi 和歌山県立医科大学, 医学部, 講師 (90609992)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 小胞体出芽部位

Outline of Final Research Achievements

Various organelles exist in cells, and microdomains, such as ER exit sites (ERES), are formed at several organelles. In this study, localization and morphology of ERES and other organelles were found to be altered under high-lactic acid conditions. The genome-wide CRISPR screening possibly screened a few candidate genes essential for such alteration of endosomes and analyzed them.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

COPII小胞は小胞体のタンパク質や脂質をゴルジ体へ向かって運ぶ輸送小胞である。COPII小胞はコートタンパク質の重合により形成されることが解明されている。一方で、COPII小胞形成部位すなわちERESやその他分泌経路のオルガネラは局在によって機能的な差があるかは不明である。本研究は未だ完全には解明されていないオルガネラ局在と機能の相関関係の解明につながると考えられる。