2022 Fiscal Year Final Research Report
Exploring lipid-modifying proteins involved in impaired insulin secretion
| Project/Area Number |
20K15977
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 糖尿病 / 脂質修飾 / クリックケミストリー |
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| Outline of Final Research Achievements |
Impaired insulin secretion is a more common cause of type 2 diabetes in Japan compared to other countries. In this study, we developed detection methods for lipid-modifying proteins involved in the transfer of insulin-containing vesicles to cell membranes in response to changes in glucose concentration.
Different chain lengths of fatty acids were synthesized as labels to analyze lipid-modifying proteins. These labels were reactive to target molecules without leaving behind residual products in vitro. Thus, we successfully modified proteins for each fatty acid length. In addition, the secretory capacity of insulin with the synthesized labels was evaluated. Finally, we attempted to develop a new analytical method for detection of modified proteins.
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| Free Research Field |
薬系分析および物理化学関連
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の学術的意義は、脂質修飾タンパク質の分析手法の開発およびインスリン分泌障害と脂質修飾タンパク質の関連性を示した点にある。まだ、不十分ではあるが、引き続き解析を続けることにより、分析化学、生化学および医学的な知見を得ることに繋がると考えている。
本研究の社会的意義は、糖尿病患者への還元が挙げられる。本邦における糖尿病患者数には有意な増減が見られていないが、世界規模では爆発的に増加している。インスリンの分泌能に関する知見を得ることは、糖尿病患者の病態理解に繋がり、発症予防・治療効果の増大に繋がる可能性がある。
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