Analysis of staphylococcal food poisoning and its emetic mechanism using a primate model

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16249
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49050:Bacteriology-related
• Research Institution: Kitasato University
• Principal Investigator: Ono Hisaya 北里大学, 獣医学部, 准教授 (80704569)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ブドウ球菌エンテロトキシン / 嘔吐型食中毒 / コモンマーモセット / 肥満細胞

Outline of Final Research Achievements

The aim of this study was to elucidate the molecular mechanism of emetic expression of staphylococcal enterotoxin (SE) and to identify the receptors involved in emesis by using the common marmoset.
In silico, we observed an interaction between SEA and a degranulation-related molecule. The protein was found to bind to SE in far western blotting. However, transfection of cultured cells did not show high affinity for SEs, and their interaction with SEs and their involvement in the induction of degranulation were unknown. To elucidate the receptors and signaling pathways involved in SE-induced mast cell degranulation, we searched for membrane proteins that interact with SE, but were unable to identify any proteins.

Free Research Field

細菌学

Academic Significance and Societal Importance of the Research Achievements

本研究がさらに進展することで肥満細胞の新規機能の解明につながると考えられる。また、SE受容体の同定によりSE受容体を恒常的に発現する肥満細胞が作出される。SE刺激により脱顆粒またルシフェラーゼ合成を行う細胞は、嘔吐モデル動物によらない嘔吐活性の評価系となるため、今後の嘔吐研究においてブレークスルーをもたらすと考えられる。