Suppression of metastasis of bone and soft tissue sarcoma by regulation of cell movement polarity via cytoskeleton

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16353
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 50010:Tumor biology-related
• Research Institution: University of Toyama
• Principal Investigator: Watanabe Kenta 富山大学, 附属病院, 診療助手 (90865255)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 細胞骨格 / 細胞運動極性 / 転移抑制

Outline of Final Research Achievements

The purpose of this study was to create a new method for inhibiting metastasis by focusing on the relationship between metastatic potential and cytoskeleton using metastatic strains with different metastatic potentials established from mouse bone and soft tissue sarcoma cells. As for the research method, we focused on the polarity of cell movement, especially in the cytoskeleton, and planned to analyze the regulation mechanism of cell movement by microtubule plus-end-accumulating proteins.
In 2D and 3D cultures, it was confirmed that eribulin, a microtubule inhibitor, reduced the directivity of cell motility, and that the principle of this was a change in the localization of APC. Next, we focused on microtubule + end-accumulating proteins such as APC, and created knockout strains and overexpressed strains, but could not establish stable cell lines.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

目的の新たな転移抑制の治療法の確立には至らなかったが、細胞運動極性の調整が転移の抑制につながる可能性は考えられた。今後安定した遺伝子操作による細胞株の樹立により、より詳細に細胞運動極性の機序、調整をすることが可能となれば現在治療困難である骨軟部肉腫の転移の抑制につながる治療法の確立を目指すことができるのではないかと考える。