Development of the killing function of iPSC derived CAR-T cell for solid tumor.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16439
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 50020:Tumor diagnostics and therapeutics-related
• Research Institution: University of Tsukuba
• Principal Investigator: Mishima Yuta 筑波大学, 医学医療系, 助教 (80770263)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: がん免疫治療 / IPS細胞 / CAT-T細胞治療 / マイクロ流体デバイス / 免疫細胞分化 / AI画像解析

Outline of Final Research Achievements

This investigation sought to identify crucial genes that enhance three fundamental capabilities associated with immune cells, namely (1) migration/invasion, which refers to the ability to move toward target cancer cells, (2) injury, which denotes the capacity to attack cancer cells, and (3) proliferation, which pertains to the ability of cells to multiply themselves.
This project aims to enhance immune cell function to treat solid tumors. To achieve this aim, I have developed a groundbreaking device capable of isolating cells that exhibit variations in these three capacities. Moreover, I have successfully validated the efficacy of the technology in isolating immune cells with diverse abilities by utilizing actual cancer cells.

Free Research Field

腫瘍免疫学、幹細胞学

Academic Significance and Societal Importance of the Research Achievements

本研究では独自に研究代表者らが開発した３つの新規技術「マイクロ流体デバイスを用いた運動機能による細胞選別方法」、T細胞と腫瘍組織との相互作用がin vitroでイメージング可能な「Tumor-on-a-chip」、「iPS細胞由来CAR-T細胞（CAR-iPS-T細胞）作製技術」をツールとして用いることにより、固形がんにおけるT細胞の遊走能力や浸潤能力の制御メカニズムの解明に取り組む。今回、免疫細胞を標的細胞への遊走性で選り分けることのできるこれまでにないデバイスの開発に成功した。これにより選別された免疫細胞を用いて、抗腫瘍効果に影響を及ぼす因子のスクリーニングができる可能性を拓いた。