Examination of the timing about transplantation of bone marrow stromal cell sheets in transected spinal cord injury in rats.

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K16501
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 51030:Pathophysiologic neuroscience-related
• Research Institution: Nara Medical University
• Principal Investigator: Keisuke Masuda 奈良県立医科大学, 医学部, 研究員 (60790376)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 脊髄再生 / 脊髄離断モデル / 骨髄間葉系幹細胞 / 細胞シート

Outline of Final Research Achievements

Due to the impact of the pandemic of COVID-19, my experiment was significantly delayed.
In the preliminary experiment, axonal regeneration and glial scar formation were evaluated by transplanting bone marrow stromal cell (BMSC) sheets into rats with a spinal cord transection model in the acute phase.
As in previous reports, CD11b-positive macrophages accumulated at the spinal cord stump, and GFAP-positive astrocytes surrounded the macrophages. It is thought that inflammatory and anti-inflammatory responses occurred in acute phase of transection-type spinal cord injury, as in compression-type spinal cord injury. In the BMSC sheet transplant group, CD206-positive M2 macrophages accumulated at the stump early on, suggesting that the BMSC sheet may promote an acute anti-inflammatory response.

Free Research Field

病態神経科学関連

Academic Significance and Societal Importance of the Research Achievements

麻痺を抱えて生活する重症脊髄損傷患者に対して現在治療法がない。現在国内外で行われている研究は基礎、臨床共に軽度から中等度の脊髄損傷に対する治療が主体であり、重症脊髄損傷に対しての研究は少ない。本研究で行う新規再生医療の開発は麻痺改善や社会復帰を可能とし、中枢神経再生領域の治療法を飛躍的に進歩させる研究である。そのため、医学に与えるインパクトは極めて大きいものと確信する。