Analysis of glymphatic system dysfunction in normal pressure hydrocephalus.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16650
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52030:Psychiatry-related
• Research Institution: Nagasaki University
• Principal Investigator: Morimoto Yoshiro 長崎大学, 医歯薬学総合研究科(医学系), 講師 (70816686)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 正常圧水頭症 / CFAP43

Outline of Final Research Achievements

First, we attempted to breed sufficient numbers of CFAP43 knockout mice in the analysis
However, 1) the probability of CFAP43 knockout mice becoming pregnant/being born is low 2) Even if they are born, they are eaten and killed by their mothers.Due to these circumstances, it was difficult to obtain a sufficient number of CFAP43 knockout mice for analysis, even after a considerable number of breeding attempts.Due to the limited number of CFAP43 knockout mice, an attempt was made to create a protocol to measure the ventricles of the brain using micro CT.However, 1) The brains and ventricles of the knockout mice were extremely small, making accurate delineation difficult. 2) Due to phenotypic differences at the individual level, there are no clear criteria for determining the degree of ventricular enlargement that constitutes "hydrocephalus".For these two reasons, it was difficult to measure ventricular enlargement in the mouse pups using micro-CT.

Free Research Field

分子遺伝学

Academic Significance and Societal Importance of the Research Achievements

上記の解析を行う中で、CFAP43ノックアウトマウスにおける以下の特徴が示唆された。(1)個体・組織・細胞レベルにおいて、表現系が均一ではないこと。(2)CFAP43遺伝子は、ハプロ不全においても何らかの表現系を来たす可能性があること。
今後のCFAP43遺伝子の機能解析および表現系発現に繋がるメカニズムを解明するにあたっては、本研究において明かとなった上記２つの課題について取り組む必要があると考えられた。