Fundamental study of radiosensitization therapy with titanium peroxide nanoparticles using exosomes

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16700
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52040:Radiological sciences-related
• Research Institution: Kobe University
• Principal Investigator: Iwashita Kazuma 神戸大学, 医学部附属病院, 医員 (80850065)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: チタンナノ粒子 / エクソソーム / APS

Outline of Final Research Achievements

Electroporation was first employed to encapsulate titanium peroxide nanoparticles (TiOxNPs) in exosomes, but the exosomes died and the TiOxNPs could not be encapsulated in the exosomes.
We focused on APS as another method of transporting TiOxNPs into the body, in which tannic acid and tannic acid are cross-linked by proteins or antibodies to form a three-dimensional structure.　We have investigated the radiosensitizing effect of APS-modified TiOxNPs. Cell survival after irradiation was lower in the group treated with APS-modified TiOxNPs than in the group treated with non-APS-modified TiOxNPs. Therefore, APS-modified TiOxNPs have a high radiosensitizing effect.

Free Research Field

放射線腫瘍学

Academic Significance and Societal Importance of the Research Achievements

我々の先行研究で開発した過酸化チタンナノ粒子は、X線照射によって大量のヒドロキシラジカルを発生し、担がんマウスを用いたin vivo実験において顕著な放射線増感効果を示すことが判明している。過酸化チタンナノ粒子は癌細胞に到達すれば放射線増感効果が期待できるが、ドラッグデリバリーの方法はまだ確立されていない。今回エクソソームを用いた運搬方法の確立は出来なかったが、APSを用いた新たな手法の確立に向けて基礎的な検討を行った。ナノ粒子を用いた新たな放射線増感療法の臨床応用に向けて引き続き検討を行う。