2022 Fiscal Year Final Research Report
The analysis of blast homing by xenotransplantation of transient abnormal myelopoiesis blast into immunodeficient mice
| Project/Area Number |
20K16858
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 52050:Embryonic medicine and pediatrics-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 一過性骨髄異常増殖症 / 急性巨核芽急性白血病 / ダウン症 / 異種移植 |
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| Outline of Final Research Achievements |
In this study, we investigated the involvement of the molecules associated with homing of TAM blasts in the development of transient abnormal myelopoiesis (TAM) associated with Down syndrome in the fetal liver or its recurrence in the bone marrow.
TAM blasts were isolated from peripheral blood of the baby diagnosed with TAM and cultured in vitro to induce EPCR as a molecule involved in interaction with fetal liver and CXCR4 as a molecule involved in homing into bone marrow. EPCR expression increased in a UM729 concentration-dependent manner, and CXCR4 expression increased in hypoxic culture.
TAM blasts unstimulated and induced to express EPCR or CXCR4 were xenotransplanted into neonatal and adult immunodeficient mice, but no TAM blast detected, respectively.
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| Free Research Field |
新生児
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| Academic Significance and Societal Importance of the Research Achievements |
研究の主たる目的である一過性骨髄異常増殖症（TAM）の発症と再発にTAM芽球のhomingに関わる分子の発現の違いが関与していることを証明することできなかった。しかし、homingに関わる分子の発現が関与していないというネガティブデータを示したことと、TAM芽球のhomingに関わる分子を誘導する培養条件を確認できたことは本研究の成果と考えている。
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