Developmental study of regenerative therapy using multi-layered cell sheet for stenosis after endoscopic treatment for superficial esophageal cancer

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K17051
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Okayama University
• Principal Investigator: Abe Makoto 岡山大学, 医学部, 客員研究員 (00819104)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 再生医療 / 消化器内視鏡治療 / 上皮細胞シート / 間葉系細胞シート / 細胞シート工学

Outline of Final Research Achievements

Mucosa regeneration therapy is attracting attention as a means of preventing complications such as gastrointestinal stenosis that may occur after mucosal resection for gastrointestinal tumors. Transplantation of epithelial cell sheets into postoperative ulcers and mesenchymal stem cell tutoring medium have been used to good effect. In this study, we succeeded in stacking epithelial cell sheets and mesenchymal cell sheets produced by cell sheet engineering in anticipation of synergistic effects on wound healing. The characteristics of the stacked sheets suggested that they could be used effectively in the pharyngeal region in human clinical practice, and preclinical studies were conducted for their clinical application.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

間葉系幹細胞を用いて製造される間葉系幹細胞シートは高い創傷治癒能力を有しているが、消化管への移植は困難であった。本研究は、同分野で既に臨床応用されている上皮細胞シートと積層化することで、同シートの移植を可能とし、さらに創傷治癒の相乗効果を期待している。同シートの積層化は既報がなく、本研究で初めて積層化に成功した。積層化シートを移植することで、咽頭領域の内視鏡治療後の嚥下機能障害および誤嚥性肺炎の発症を抑制することが期待できる。