2023 Fiscal Year Final Research Report
Development of bacteriophage therapy for normalisation of bacterial flora and treatment of diseases.
| Project/Area Number |
20K17060
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53010:Gastroenterology-related
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| Research Institution | Azabu University (2022-2023)
Jichi Medical University (2020-2021) |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | クローン病 / バクテリオファージ / ファージセラピー / 細菌叢 / 難病 |
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| Outline of Final Research Achievements |
Crohn's disease (CD) is one of the designated intractable diseases and is characterized by chronic inflammation of the digestive tract. Dysbiosis of the gut microbiota is involved in its onset, with AIEC being particularly implicated. However, the treatment guidelines do not include therapeutic approaches targeting the microbiota. The applicant hypothesized that using bacteriophages to eliminate AIEC from the microbiota could lead to disease treatment. Experiments with mice confirmed the removal of AIEC and improvement of disease symptoms, and the CRISPR target specificity for AIEC-specific genes was analyzed. These findings demonstrated the potential of synthetic phage therapy targeting the microbiota, indicating a new treatment pathway for Crohn's disease.
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| Free Research Field |
細菌学
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| Academic Significance and Societal Importance of the Research Achievements |
ヒト腸管には細菌が棲みついおり、健康維持に重要である。しかし、この細菌叢が乱れると病気の原因となる。治療のために細菌叢を正常化する方法が注目されているが、既存の手法には確実性などの問題があり、臨床への展開が遅れている。この研究では細菌叢中に微量ながら存在するが他の細菌を支配し、細菌叢を破綻状態に追い込む種に着目した。この原因菌を細菌のウイルスであるバクテリオファージを用いて特異的に除去することで細菌叢を正常化し、難病モデルにおいて治療効果があることを確認した。
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