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2023 Fiscal Year Final Research Report

Proteomics of serum exosome to identify a novel biomarker for bronchial asthma

Research Project

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Project/Area Number 20K17180
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 53030:Respiratory medicine-related
Research InstitutionOsaka University

Principal Investigator

Koba Taro  大阪大学, 大学院医学系研究科, 招へい教員 (00812942)

Project Period (FY) 2020-04-01 – 2024-03-31
Keywords気管支喘息 / エクソソーム / バイオマーカー / プロテオミクス
Outline of Final Research Achievements

We have identified novel biomarkers related to the pathogenesis of bronchial asthma by comprehensively analyzing exosomes (extracellular vesicles) floating in the blood of bronchial asthma patients, identifying a vast number of proteins (more than 3000 kinds). Additionally, we integrated comprehensive analysis of bronchial asthma patients' lungs. In particular, biomarker molecules such as galectin-10 are not only useful for asthma diagnosis and predicting airflow obstruction but also correlated with etosis (eosinophil cell death), which is closely related to the pathophysiology of asthma. Although blood eosinophil count has been mainly utilized as a biomarker for asthma, galectin-10 in exosomes was suggested to be a novel biomarker superior to eosinophil count.

Free Research Field

プロテオミクスによる呼吸器疾患のバイオマーカー探索

Academic Significance and Societal Importance of the Research Achievements

気管支喘息は、症状、気道過敏性や気道可逆性により総合的に診断される疾患で、血液検査では血中好酸球やIgE等により、肺内の病態を推測しながら治療法を検討しているものの、肺内の疾患活動性を反映する分子を血液で捉えるには限界があった。我々は、血中の細胞外小胞であるエクソソームに着目し、最新プロテオミクス(蛋白網羅的解析)を駆使することで新たな気管支喘息バイオマーカーの一つとしてガレクチン10を同定した。ガレクチン10を含む新規バイオマーカー分子は、通常、喘息の診断において、ゴールドスタンダードとされる末梢血好酸球数に診断能で優るだけでなく、気流閉塞や粘液栓とも有意な相関を認めた。

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Published: 2025-01-30  

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