Strategies to innovate novel microbiome-based therapies for inherited keratinization disorders

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17316
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53050:Dermatology-related
• Research Institution: Nagoya University
• Principal Investigator: Murase Chiaki 名古屋大学, 医学系研究科, 客員研究者 (10846611)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 遺伝性角化異常症 / 皮膚細菌叢 / 皮膚バリア機能障害

Outline of Final Research Achievements

We analysed a variety of methods to extract DNA from the microbiota on the skin, and performed the extraction using the most efficient method. 16S rRNA analysis was performed on the collected DNA through MiSeq, to determine the microbiomic status of each case. By investigating the genetic information of microbiota as a whole, we were able to understand the differences and characteristics associated with disease and clinical symptoms. Although we were able to perform 16S rRNA analysis from samples from a wide range of diseases, unfortunately we were unable to collect an adequate number of samples for each disease, and hence it was difficult to come to an overall conclusion. We aim to continue to collect samples and analyse data to compare the microbiota between cases of the same disease, and also between different diseases.

Free Research Field

遺伝性角化異常症　皮膚軟部組織感染症　皮膚バリア機能障害

Academic Significance and Societal Importance of the Research Achievements

本研究では遺伝性角化異常症において，16S rRNA解析の手法を用いて皮膚マイクロバイオームの解析を行うことで，細菌叢特異的治療法の開発に直結する基礎的データを得ることを目的とした。外界から皮膚を守る皮膚バリア機能に生まれつき障害を有する，遺伝性角化異常症において，皮膚細菌叢（マイクロバイオーム）が与える影響に着目した。遺伝性角化異常症患者の皮膚では，健常人の皮膚と比較して，バリア機能の障害から皮膚炎を起こしやすく，細菌の侵入が容易となるため皮膚感染症に罹患しやすい。臨床症状の変化に伴う皮膚マイクロバイオームの変化を観察・評価することは，将来的な細菌叢特異的治療法への応用への基礎となる。