2022 Fiscal Year Final Research Report
Study on the migration capacity of type 2 innate lymphocytes in inflammatory skin diseases
| Project/Area Number |
20K17331
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53050:Dermatology-related
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
Nagai Makoto 兵庫医科大学, 医学部, 講師 (30791545)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ２型自然リンパ球 / ILC２ / アトピー性皮膚炎 |
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| Outline of Final Research Achievements |
We previously generated a transgenic mouse line expressing skin-specific IL-33 (IL33tg mice) and showed that IL-33 elicits group 2 innate lymphoid cell (ILC2)-dependent atopic dermatitis-like skin inflammation.We sorted ILC2s from the skin and draining lymph nodes of IL33tg mice and analyzed their transcriptomes using the single-cell RNA sequencing technique, which revealed that the skin ILC2s had split into two clusters: circulating ILC2 and skin-resident ILC2. We tracked ILC2 migration using IL33tg-Kikume Green-Red mice. Exposing the IL33tg-Kikume Green-Red mice’s inflamed skin to violet light allowed us to label the circulating ILC2s in their skin and track the ILC2 migration from the skin to the draining lymph nodes.
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| Free Research Field |
２型自然リンパ球
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| Academic Significance and Societal Importance of the Research Achievements |
IL-33は２型自然リンパ球を活性化する物質で、皮膚にIL-33が過剰になる遺伝子改変マウス（hK14mIL33tg）はアトピー性皮膚炎になります。しかし、ILC2が皮膚からリンパ節に移動するかどうかは、不明でした。この研究では珊瑚（キクメイシ）の遺伝子をマウスの細胞に入れることで皮膚ILC2の動態を可視化し、皮膚病変部から所属リンパ節にILC2などの免疫細胞が遊走する動態を明かにします。本研究によって、ADにおけるILC2のリンパ節への遊走能とその意義が明らかになることで、アレルギー性疾患の新規治療法の開発や創薬に繋がることが期待されます。
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