Elucidation of adjustment mechanisms of reactive oxygen species by chlamydial infection

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K17470
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 54030:Infectious disease medicine-related
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: YAMAZAKI Tomohiro 北海道医療大学, 医療技術学部, 講師 (10784829)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 性器クラミジア / 活性酸素

Outline of Final Research Achievements

Chlamydia trachomatis is one of the main causes of sexual transmitted disease worldwide. On the other hand, the increased production of reactive oxygen species (ROS) thought to be involved in the development of cancer cells, and it has been reported that C. trachomatis modulates ROS production in infected host cells. It is considered that regulation of the expression of NADPH oxidase (Nox) is involved in chlamydial ROS regulation. Therefore, in this study, we investigated the expression of Nox during chlamydial infection, but no significant difference was found. However, it was confirmed that Nox inhibitor and other ROS regulating reagents suppress the chlamydial growth. Thus, it was suggested that intracellular ROS production may have some influence on the growth of C. trachomatis.

Free Research Field

病態解析学

Academic Significance and Societal Importance of the Research Achievements

本研究では、Nox阻害剤や他のROS調節試薬を使用することで性器クラミジアの増殖に影響を与えることを明らかにした。これにより、細胞内ROS産生が性器クラミジアの細胞内増殖に何らかの影響を与えている可能性が示唆された。今後さらなる研究により、性器クラミジアの感染細胞内でのROS調節機構を明らかにすることで、性器クラミジアの病態形性機構の解明に繋がり、感染拡大の防止に貢献できるのではないかと考えている。