Diagnostic Method for Treatment Resistance of GIST by ctDNA Monitoring Using an PVA Sponge

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K17614
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Osaka University
• Principal Investigator: Yamashita Kotaro 大阪大学, 医学部附属病院, 助教 (20747159)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: ctDNA / GIST

Outline of Final Research Achievements

Last year, we conducted a feasibility study of real-time PCR using the PVA sponge method. We examined the condition setting of real-time PCR using the PVA sponge method, and developed a more stable and quantitative column method. In addition, we developed a direct method for efficient measurement of a large number of samples in a real clinical setting.
In this year, we further demonstrated the superiority of the PVA sponge method (column method) over existing cfDNA assay kits using blood samples from healthy subjects, GIST patients, and cancer patients, and also demonstrated that the PVA sponge method is more efficient in collecting cfDNA than the existing cfDNA assay kits, and that the PVA sponge method can be used with several sizes of cfDNA samples regardless of the size of the samples. The method was shown to be capable of recovering and measuring cfDNA.

Free Research Field

上部消化管

Academic Significance and Societal Importance of the Research Achievements

本研究開発により、簡便な方法で血液中のcfDNAやctDNAが回収、測定、解析可能となれば、今回対象としたGISTのみならず他の癌腫を対象とした癌の存在診断や再発診断に有用である可能性が高く、多くの国民やがん患者にとってその学術的意義や社会的意義が高いと考えられる。