2021 Fiscal Year Final Research Report
Development of an Artificial Circular RNA Medicine for overcoming treatment resistance in Esophageal Squamous Cell Carcinoma
| Project/Area Number |
20K17683
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 55020:Digestive surgery-related
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | 環状RNA / 食道癌 |
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| Outline of Final Research Achievements |
The purpose of this study is to identify circRNAs associated with treatment resistance in esophageal squamous cell carcinoma.
We analyzed the ESCC cell line TE11 and the established TE11 cisplatin-resistant line (TE11R) by next-generation sequencing to identify circX associated with CDDP resistance. It was also confirmed that knockdown of circX enhanced drug sensitivity. In addition, we quantified CircX expression in the tissues of 46 patients with esophageal squamous cell carcinoma who underwent surgical resection after cisplatin-containing chemotherapy, and found a correlation between high CircX expression and treatment resistance in pretreatment biopsy tissues, suggesting that CircX may be a factor involved in treatment resistance.
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| Free Research Field |
消化器外科
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、食道扁平上皮癌と関連するcircRNAを特定することができた。これまで、circRNAを用いた核酸医薬は未だ開発されていないが、circRNAのその安定した構造と、転写翻訳を制御する機能を有する点に着眼し、核酸医薬の次世代の主役として期待される。今後は、特定されたcircRNAを用いたあらたな核酸医薬の開発につながるとかんがえられる。また、本研究により食道癌におけるCDDP抵抗性に関与するcircRNAを同定することでき、あらたなバイオマーカーとなる可能性を秘めており、その恩恵は多大であると考えている。
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