Investigating the Cause of Hemolysis in Patients Supported by a Pulsatile Ventricular Assist Device

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17719
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55030:Cardiovascular surgery-related
• Research Institution: Kyushu University
• Principal Investigator: Harada takeaki 九州大学, 医学研究院, 共同研究員 (40644703)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 左室補助人工心臓 / 溶血 / 粒子画像流速測定法 / せん断応力

Outline of Final Research Achievements

Using the test circuit, we were able to confirm that hemolysis actually occurred in the AB5000 under conditions that deviated significantly from those of the AB5000 and NIPRO VAD in the speciality evaluation experiment using fresh ACD-added bovine blood.Furthermore,we measured particle velocity and shear velocity near the valve using particle image velocimetry under these conditions,and were able to confirm that shear stress was not strong enough to cause hemolysis as the cause of hemolysis.Shear stress as a cause of hemolysis could not be the sole cause of hemolysis. The magnitude of negative pressure and the time of negative pressure application were confirmed to be involved in hemolysis, a fact that has not been reported before.

Free Research Field

心不全

Academic Significance and Societal Importance of the Research Achievements

補助人工心臓を使用するにあたり溶血を生じると様々な程度の急性腎障害を伴いさらに播種性血管内凝固症候群を伴う事もある。この事からも溶血症は実臨床において非常に大きな合併症と考えられる。そのためそれぞれの駆動機の構造を含めてAB5000とニプロVADの拍動流型VADの特性や溶血の発生する条件を比較検討することで溶血の原因、その機序を明らかにし実臨床の場で応用し溶血を減らす事は出来ると考えられる。また今後を拍動流型VADを使用する際に合併症を少なくし安全に有効に使用可能にする事が本研究の最終的な目的である。