Possibility of lung injury repair by intratracheal administration of porcine fetal lung mesenchymal stem cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17752
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55040:Respiratory surgery-related
• Research Institution: The University of Tokushima
• Principal Investigator: TAKASHIMA Mika 徳島大学, 病院, 特任助教 (70596254)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 肺再生 / ブタ胎仔肺 / 間葉系幹細胞 / LPS誘導急性肺障害 / 気道内投与

Outline of Final Research Achievements

To extract mesenchymal stem cells (MSCs) derived from porcine fetal lung, 54-day-old fetal porcine lung tissue was excised. After the culture treatment, the cells were centrifuged, and the isolated mononuclear cells were cultured. In order to prove that these cells are MSCs, a group of cells positive for mesenchymal markers (D29, CD44, CD90, CD105) and negative for hematopoietic markers (CD34, CD45) was extracted by flow cytometry. It proved that the cells were MSCs. In addition, we intratracheally administered these MSCs to LPS-induced acute lung injury model pigs, and confirmed engraftment of the administered MSCs.

Free Research Field

呼吸器外科学

Academic Significance and Societal Importance of the Research Achievements

ブタ胎仔肺組織から旺盛な増殖能と多能性を持つとされる胎仔肺由来の間葉系幹細胞を抽出する手技を確立することができた。またこれらの細胞をLPS誘導急性肺障害モデルブタに投与する手技を確立することができた。本細胞を用いることで、今後の肺再生の基礎的知見を得ることが期待できると考える。