2022 Fiscal Year Final Research Report
Ultrastructural Alteration of Pulmonary Tissue under High Concentration Oxygen Condition
| Project/Area Number |
20K17857
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 55060:Emergency medicine-related
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 高濃度酸素 / グリコカリックス / 微小循環 / 肺障害 |
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| Outline of Final Research Achievements |
To determine the structure of pulmonary tissue under conditions of high oxygen concentrations.Ten-week-old C57BL male mice and control mice were exposed to 100% oxygen and to room air for 72 hours, respectively. Lung specimens obtained from these mice underwent morphological analysis and immunofluorescence studies. We used electron microscopy to determine the ultrastructure of the pulmonary capillaries, including the endothelial glycocalyx. Perivascular cavity enlargement was detected 12 hours after 100% oxygen administration and expanded over time. Ultrastructural analysis using electron microscopy revealed collapsed alveoli and pulmonary capillary wall and alveolar wall thickening in the 100% oxygen group. The pulmonary capillary endothelial glycocalyx was injured in the 100% oxygen group. High-concentration oxygen may be involved in pulmonary endothelial glycocalyx injury.
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| Free Research Field |
救急・集中治療
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| Academic Significance and Societal Importance of the Research Achievements |
酸素療法は最も効果的な医療介入の一つであるが、高濃度酸素供給は内皮グリコカリックスの劣化を介した微小循環障害を引き起こす。逆に、内皮グリコカリックスの保護は、高濃度酸素供給による肺の傷害を軽減する可能性があり、今後の発展が期待される。
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