Development of intestinal environment design for severe pathological conditions using metagenomic and metabolomic analysis

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K17861
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Osaka University
• Principal Investigator: Muratsu Arisa 大阪大学, 医学部附属病院, 医員 (90737199)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 腸内細菌 / 代謝産物

Outline of Final Research Achievements

In severe emergency conditions such as sepsis, it has been shown that disruption of the intestinal microbiota leads to a systemic inflammatory response. However, an integrated and comprehensive analysis of intestinal bacteria and metabolites has not been performed. In this study, we used metagenomic and metabolomic analyses to elucidate the intestinal microbiota and metabolites in a mouse model of sepsis and to explore potential factors contributing to systemic inflammation.
Our study showed that Enterobacteriaceae increased in the acute phase after invasion, and Lachnospiraceae and Ruminococcaceae families increased in the subacute phase. Branched-chain amino acids and Choline increased, while short-chain fatty acids decreased.

Free Research Field

救急医学

Academic Significance and Societal Importance of the Research Achievements

侵襲後、腸内細菌叢と代謝産物は大きく変化していることが示された。今後増減する腸内細菌や代謝産物が病態へと与える影響を評価することで、重症救急病態に対する新たな治療法の開発のために役立つ可能性がある。