Pancreatic Endothelial Disorder in Acute Pancreatitis

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17888
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Gifu University
• Principal Investigator: Yasuda Ryu 岐阜大学, 大学院医学系研究科, 招へい教員 (70869354)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 急性膵炎 / 好中球 / 好中球コロニー刺激因子

Outline of Final Research Achievements

This study aimed to investigate the role of neutrophils in pancreatitis by examining tissue dynamics. We created a model of cerulein-induced pancreatitis in 12-week-old male neutrophil colony-stimulating factor knockout mice (G-CSF-KO) and wild-type littermate control mice by intraperitoneal administration of cerulein at a dose of 80 ug/kg every hour for six consecutive days. Mice were sacrificed 3, 6, 12, 24, 36, 48, 72, and 168 h after cerulein administration and examined histologically. The survival rate after one week of cerulein administration was 100% in the control mice, whereas it was significantly lower (10%) in the G-CSF-KO mice. Histological examination revealed significant hemorrhage and inflammatory cell migration in the G-CSF-KO mice, indicating prolonged inflammation. Prolonged inflammation was observed in the G-CSF-KO mice. Tissue cleanup by neutrophils during the acute phase of inflammation may influence healing through the chronic phase.

Free Research Field

救急・集中治療

Academic Significance and Societal Importance of the Research Achievements

急性膵炎は、病的に活性化した膵酵素と好中球などの炎症細胞が二次的に産生する炎症メディエーターによって引き起こされる無菌性の炎症であり、最も治療が難しい疾患の一つであるため、本研究によりその機序が解明されることは社会的意義が大きいと考えられる。