Gene expression analysis of affected blood vessels in moyamoya disease

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K17961
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56010:Neurosurgery-related
• Research Institution: Nagoya University
• Principal Investigator: Yokoyama Kinya 名古屋大学, 医学部附属病院, 助教 (90867904)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: moyamoya disease / transcriptome / Microarray / messenger RNA / long non-coding RNA

Outline of Final Research Achievements

During the study period, 21 patients with Moyamoya disease, 6 patients with aneurysms and 5 patients with intractable epilepsy were sampled from the brain surface arteries and subjected to comprehensive gene expression analysis using microarrays. Comprehensive gene expression analysis for messenger RNA was performed on 11 moyamoya disease patients, 6 aneurysms as a control group, and 3 intractable epilepsy patients. In addition, we comprehensively analyzed long non-coding RNA expression in the intracranial artery walls of 21 patients with moyamoya disease and 11 patients with non-moyamoya disease, and published the results in a paper.

Free Research Field

脳神経外科分野

Academic Significance and Societal Importance of the Research Achievements

もやもや病の網羅的遺伝子発現解析については、これまで循環血中やiPS細胞については行われてきたが、病変部血管に対するものとしては我々の研究が初となった。これまでの研究では炎症や免疫応答の関与が推察されてきたが、今研究により、もやもや病の病変部である、脳動脈血管壁においても、同様の変化が生じていることが示された。