Identification of non-invasive miRNA biomarkers for ccRCC by comprehensive analysis of urinary exosomes

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K18091
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56030:Urology-related
• Research Institution: Osaka University
• Principal Investigator: wang cong 大阪大学, 医学系研究科, 招へい教員 (70783893)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 腎癌 / エクソソーム / miRNA

Outline of Final Research Achievements

We focused on miRNAs of urinary exosomes with the aim of identifying novel biomarkers of clear cell renal cell carcinoma (ccRCC). We extracted exosomes not only from urine, but also directly from surgically resected tissue samples by ultracentrifuge methods in order to detect cancer-specific urinary exosomal miRNAs. Microarray analyses were conducted. 8 candidates miRNAs extracted from tissue samples were found to be significantly higher in ccRCC patients and 12 candidates miRNAs were identified significantly higher from urine samples. As the candidates from the two groups were different from each other, we could not identified cancer-specific exosomal miRNAs from urine in ccRCC, and further studies are needed in this area.

Free Research Field

腎癌

Academic Significance and Societal Importance of the Research Achievements

我々は確立した組織培養由来エクソソーム抽出法を用いて、今回腎癌組織および正常組織からそれぞれ特異的なエクソソームを単離することに成功し、さらに内包miRNAの抽出も可能であることを検証できた。また腎癌患者および健常者尿中エクソソームの回収単離にも成功し、尿中エクソソーム内包miRNAの解析も成功した。今後他癌種への応用も可能と考え、さらに研究の幅を広げることにより、癌特異的なマーカーの探索に役に立つことが期待できる。