Elucidation of metastic invasion in ovarian serous carcinoma

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K18229
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Dokkyo Medical University
• Principal Investigator: Yamaguchi Noriko 獨協医科大学, 医学部, 助教 (50755618)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 卵巣癌 / miR-223 / 上皮間葉転換 / ZEB-1

Outline of Final Research Achievements

Epithelial-mesenchymal-transition (EMT) plays essential roles in ovarian cancer invasion, metastasis, and drug resistance. The aim of this study is to clarify whether miR-223 regulates EMT through ZEB-1 in ovarian serous carcinoma. Expression of miR-223 and ZEB-1 were analyzed by immunoblotting and real-time RT-PCR analysis. Morphological change, invasion ability, and chemosensitivity were evaluated by using2 miR-223 stable over expression cell line in OV1063. The correlations between tmiR-223 and ZEB-1 were determined. miR-223 may regulate EMT through ZEB-1 degradation in ovarian serouscarcinoma and might be a predictive marker for a favorable prognosis in the treatment of this cancer.

Free Research Field

婦人科腫瘍学

Academic Significance and Societal Importance of the Research Achievements

今後卵巣漿液性癌の臨床病理学的因子とmir-223の相関関係が明らかとなれば、実際に予後予測や早期診断への応用や病勢把握が実現する可能性がある。特に卵巣癌は検診の意義が確立されておらず、発見時進行していることが多い為、これが診断マーカーとして確立すれば早期発見につながる可能性もある、更に、近年発展しているmicroRNA創薬にトライすることで新たな治療戦略の道が開ける可能性もある。